Assay of Antinuclear Antibodies by ELISA Using Nuclei as Antigen

Assay of antinuclear antibodies by ELISA using nuclei as antigen.

An enzyme-linked immunosorbent assay to detect antinuclear antibodies in the sera of patients with autoimmune diseases is described. Goat liver nuclei were immobilized on polystyrene plates and antinuclear antibodies were used to standardize the assay. The effects of variables, such as the nuclei concentration, conditions of nuclei storage, and the length of the incubation period were investiga...

Immunodiagnosis of Human Fascioliasis by Elisa Using Excretory–Secretory Products as Antigen

Background: Different immunological methods are used for the serodiagnosis of Fasciola hepatica infections, however, they have limited specificity and/or sensitivity.Objective: To improve the specificity of ELISA using a partially modified method for preparation of excretory-secretory (ES) antigens.Methods: Studied groups were as follows: 75 patients with parasitological evidence of fascioliasi...

FMDV antigen-detection ELISA using multiserotype-reactive monoclonal antibodies

The Use of Poly-L-Lysine as a Capture Agent to Enhance the Detection of Antinuclear Antibodies by ELISA

Antibodies to nuclear antigens (antinuclear antibodies or ANAs) are the serological hallmark of systemic lupus erythematosus (SLE). These antibodies bind diverse nuclear antigens that include DNA, histones and non-histone proteins as well as complexes of proteins with DNA and RNA. Because of the frequency of ANA expression in SLE, testing is an important component of clinical evaluation as well...

anti deoxyribonucleic acid and antinuclear antigen antibodies in graves’ disease

graves’ disease is an autoimmune disorder characterized by presence of antibodies directed against thyroid stimulating hormone (tsh) receptor or nearby region. other serological abnormalities like antibodies to double stranded dna (ds–dna) and antinuclear antibodies (ana) have also been observed. we studied antibodies to ds-dna and ana in our patients with graves’ disease and compared them with...